Any bias on behalf of the ants, which reside in the United Kingdom, was not taken into account, but this does not worry Mr Nicholson who said he was "forever hopeful" of England's chances of success.
But the England manager was not happy with the press who snapped the photo.
"We want to show people an England team can play in a different way".
Gareth Southgate was hailed for "playing a blinder" ahead of England's World Cup opener with Tunisia, as the afterglow of a notionally happy squad and not-quite-as-adversarial-and-vituperative-media was directed his way.
He then doubled down by claiming, "Our media has to decide if they want to help the team or not".
The team note, which conveys a majority of a potential starting XI for Sunday's World Cup group stage clash against Panama, implies that Marcus Rashford will be in the first team alongside Harry Kane up top.
Four years ago in Brazil, they failed to make the knockout rounds after picking up just a point, and at South Africa 2010, the Three Lions needed a 1-0 win against Slovenia to scrape into the last 16.
"There's no drama for me about it".
Gareth Southgate's side beat Tunisia 2-1 in their opening game but missed a series of first-half chances and needed a stoppage-time victor from captain Harry Kane to secure the three points.
"So, we're not going to be wildly different to that".
"We've seen already the difficulty big countries, in terms of rankings, have had in terms of breaking down lower-ranked teams". We will do our job against any team we face. "That's been a theme throughout so there will be no complacency in the way we've prepared for the game", he said.
We have leaf cutter ants in one glass box and they get their food in another.
"Obviously any time if we were to give the opposition the opportunity of having our team it's a disadvantage to us", Southgate told reporters, according to FourFourTwo.
"I feel they need to worry about us more than we need to worry about them".
The guys have to find stories, produce content, we have to get results, but it's a bit like when you go on holiday with your family - after you've been away a while, you're a bit sick of some people and less so of others.