The ex-Trinidad & Tobago worldwide also played for England U21s and youth team levels.
Samuel was killed in a auto crash on Tuesday morning in the north west of England, the Trinidad and Tobago Football Association announced in a statement.
The 37-year-old from Leigh, who played for Aston Villa and Bolton Wanderers in the Premier League and represented the Trinidad and Tobago global team, was driving a Range Rover that was involved in a collision with a van on West Lane at about 7.55am on Tuesday.
"Sadly the driver of the auto, Jlloyd Samuel, 37, from Lymm, died at the scene", they said in a statement.
Cheshire police confirmed Samuel died on the scene.
They added: "His next of kin have been informed and are now being supported by specially trained officers". "He represented Trinidad and Tobago on two occasions and on both occasions with distinction", John-Williams said.
Cheshire Police also said the driver of the van, a 54-year-old man, sustained serious injuries and has been taken to hospital for treatment.
It added: "The Trinidad and Tobago Football Association and his former national teammates at this time extends deepest condolences to his family members both in the United Kingdom and here in Trinidad and Tobago".
Football players who knew Samuel took the chance to send condolence messages through social media. One of them was Ashley Young, who played with Samuel at Aston Villa a while back.
He played youth football for Charlton before joining Aston Villa, where he made 199 appearances between 1999 and 2007.