Three full planes, each carrying 220 passengers, are flying to Enfidha this week after the Foreign and Commonwealth Office eased its travel advice.
As a result Thomas Cook resumed Tunisian holiday sales to United Kingdom customers and flight MT1800 set off from Birmingham to Hammamet/Enfidha at 6.05am today.
Earlier in 2015, former Black Country solicitor Sally Adey, who lived near Albrighton, was killed in an Islamic State attack at the Bardo Museum in the Tunisian capital Tunis.
Some 440,000 people from the United Kingdom visited Tunisia in 2014, according to the Office for National Statistics.
The ban was caused by a terrorist attack that took place a few years ago in the coastal city of Sousse.
Government travel advice reads: "Since the terrorist attack in Sousse in June 2015, which targeted tourists, the United Kingdom government has been working closely with the Tunisian authorities to investigate the attack and the wider threat from terrorist groups".
"The Tunisian government has improved protective security in major cities and tourist resorts". More flights will be added in April and May with new departure points, including Newcastle and Glasgow.
British tourists lay floral tributes on the beach after the attack.
Meanwhile Metropolitan Police counter-terrorism detectives are said to have been working with resort staff to help them spot any suspicious activity.
In reply to a tweet by BBC Breakfast showing an interview with a Thomas Cook official, Colin McCulloch wrote: 'Great. I think I'll pass on that'.
Once this was updated, Thomas Cook resumed holiday sales to people in the United Kingdom in August, with trips starting today.
"Its soft, long beaches, year-round sun, historic ruins and tasty local food mean it attracts lots of loyal visitors, as well as those visiting for the first time".
TUI Group, the operator with whom the victims had travelled, said last month it too planned to offer holidays in Tunisia again, starting in May.
While Thomas Cook's chief executive Peter Fankhauser said it had done everything it could in terms of security.