As Bolt fell to the ground, the leg with the golden shoe giving way, the crowd still went wild because the home team went on to win gold in 37.47 seconds, .05 seconds ahead of the United States.
Kevin Mayer of France extended his lead in the decathlon after the pole vault, clearing 5.10 meters in the eighth of 10 events at the world championships.
Not the way Bolt would want to end his career, but he's still the greatest sprinter of all time.
Gatlin defeated Bolt in the 100-meter relay last weekend, smoking the competition after finishing the race at 9.92 seconds to grab the gold medal at the World Athletic Championships. Another German, Kai Kazmirek, is in bronze-medal position with 7,796 points. (Bolt's injury) just happened.
The top three from each of the two heats and next two fastest finishers from the remaining qualify for the final round.
As expected, the United States also qualified.
Usain Bolt last race live streaming online: Jamaican legend Usain Bolt will take part in his last ever race in London at the World Championship. The Americans were seen as the favourites, with Bolt showing signs of weakness in the individual 100 meters and most expecting him to start his leg with a large deficit. Britain gladly bagged a silver in 42.12, and defending champions Jamaica clocked 42.19 for the bronze.
Johannes Vetter of Germany won the javelin title with an opening throw of 89.89 meters.
Eight-time Olympic champion Bolt bows out of athletics having also won 11 world gold medals but failed to add to that tally in London this summer having also surrendered his 100m crown to Justin Gatlin - a race he finished third in.
It was a remarkable comeback for the Australian as she had suffered two years of injury, missing both the 2015 world championships and the Olympics last year.
Yohan Blake was the first of the Jamaican's through the media mixed zone, slamming his fists on a barrier in front of two Japanese reporters who looked completely unsure if they wanted to provoke him further with a question.
He edged out American Paul Chelimo in a desperate dash for the line, the last mile of a hitherto slow run race run in just under four minutes.