The cutting of all transport ties by Saudi, the Emirates and Bahrain are expected to have a knock-on effect on Qatar's economy and hamper preparations for the football World Cup, due to take place in the country in 2022.
Kuwait's Emir, Sheikh Sabah Al-Ahmad Al-Jaber Al-Sabah, urged Qatar's Emir on Monday to calm tensions with allies and refrain from escalating the diplomatic rift among Gulf Arab states, according to the Kuwait News Agency.
Saudi Arabia, the UAE, Bahrain and Egypt announced the suspension of all flights to and from Qatar as of Tuesday morning. PIA officials in Qatar have also been directed to contact the Pakistani embassy in Doha.
Travel disruption is expected as the airport in Doha, Qatar's capital, is a major hub for worldwide flight connections. It said it ordered shipping agents not to receive any vessels owned by Qatari companies or Qatari nationals and not to unload any goods from Qatar.
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Members of the Gulf Cooperation Countries signed a memorandum of understanding with the USA to make it illegal in their countries for raising funds for terrorist and extremist organisations.
2010 - worldwide soccer body Federation Internationale de Football Association selects Qatar to host the 2022 World Cup despite concerns over the conditions of laborers in the country.
The three Gulf countries and Egypt accused Qatar of supporting terrorism and destabilizing the region.
For Saudi Arabia as well, Qatar's relationship with the Muslim Brotherhood (MB) might be an issue as it has also declared the MB as a terrorist organization. "... Taking all legal measures in collaboration with friendly countries and global companies with regards to Qataris using the UAE airspace and territorial waters, from and to Qatar, for national security considerations", it had said in a statement.
All the four UAE airlines including Emirates, Etihad Airways, flydubai and Air Arabia have suspended flights to and from Doha.
Sheikh Mohammed said that the Emir of Kuwait played a big role in the 2014 crisis.
The United Arab Emirates said on Tuesday that Qatar would need to provide a "guaranteed roadmap" before it would consider mending ties. They also say its authorities have supported both ISIS and al-Qaida.
The foreign minister pointed to the campaign that was aimed at Qatar in Western capitals and the United States, expressing regret that some GCC ambassadors are carrying out a campaign of incitement and distortion of Qatar's reputation contrary to the basic charter of GCC and the social cord between GCC states.
Still, a 40-year-old Egyptian manager of an audit firm told The Associated Press by phone from Doha that "the situation of Egyptians in Qatar has never been affected by political relations before". They are tracking the Al Ruwais LNG tanker, which is nearing the waterway and plans to become the first Qatari cargo to pass through since the row erupted.